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Hitachi Chemical Diagnostics, Inc.

ExoComplete™ Tube Kit

Description

Isolated EMVs can be lysed directly on the filter material by adding lysis buffer. Guanidine thiocyanate and other components of the buffer immediately and effectively inactivate ribonucleases to ensure isolation of intact mRNA. After transferring the EMV lysates to the mRNA capture plate, mRNA can be isolated through hybridization with single-strand oligo (dT) immobilized in the wells of the mRNA capture plate. For cDNA synthesis applications, antisense primers of target genes can be added to the lysis buffer. The antisense primers hybridize to the target mRNA and maximize the efficiency and specificity of the reverse transcription reaction.

Tube Kit Contents

Kit includes:

  • (12) Collection Tubes**
  • (4) mRNA Capture Plates
  • Reagents

*Starter kit also available (#88010)
**Additional tubes sold separately (#88004)

Downstream applications

  • mRNA isolation
  • Single- and double-stranded cDNA synthesis
  • Real-time quantitative RT-PCR (qPCR)
  • Next-generation RNA sequencing

Published Research Applications

Urine Exosome mRNA in Diabetic Kidney Disease (DKD)

Using the ExoComplete Tube Kit, researchers were able to identify several potential DKD biomarkers. Exosome mRNA was purified from healthy and DKD patients using the kit and prepared for NGS. Differential gene expression analysis identified 27 mRNA that were up-regulated in DKD patients. RT-qPCR validated 6 of 27 potential biomarkers.1

*1
Yamamoto, Cindy et. al. Urine Exosome mRNA in Diabetic Kidney Disease. ISEV 2015 Poster Presentation. April 2015

Specifications

Sample Volume

  • ExoComplete Tube Kit
    ≤ 12.5 mL per tube

Storage

  • Ambient

Regulatory Approvals

  • For Research Use Only

Disclaimer

The ExoComplete™ 96-Well Plate Kit and ExoComplete™ Tube Kit are Research Use Only Products. Neither of these products are intended for the diagnosis, prevention, or treatment of a disease, nor have they been validated for such use either alone or in combination with other products.