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Hitachi Chemical Diagnostics, Inc.

ExoComplete™ 96-Well Plate Kit

Description

Isolated EMVs can be lysed directly on the filter material by adding lysis buffer. Guanidine thiocyanate and other components of the buffer immediately and effectively inactivate ribonucleases to ensure isolation of intact mRNA. After transferring the EMV lysates to the mRNA capture plate, mRNA can be isolated through hybridization with single-strand oligo (dT) immobilized in the wells of the mRNA capture plate. For cDNA synthesis applications, antisense primers of target genes can be added to the lysis buffer. The antisense primers hybridize to the target mRNA and maximize the efficiency and specificity of the reverse transcription reaction.

Well Plate Kit Contents

Kit includes:

  • (4) Exosome Isolation Plates
  • (4) mRNA Capture Plates
  • Reagents

*Starter kit also available (#88009)

Downstream applications

  • mRNA isolation
  • Single- and double-stranded cDNA synthesis
  • Real-time quantitative RT-PCR (qPCR)
  • Next-generation RNA sequencing

Published Research Applications

Plasma EMVs in Post-Transplantation Bone Marrow Assessment

Plasma EMVs in Post-Transplantation Bone Marrow Assessment Using the ExoComplete 96-Well Plate Kit, researchers were able to identify potentially new biomarkers for the assessment of bone marrow condition. Exosome mRNA was purified from patients before and after undergoing hematopoietic stem cell transplantation. Hematopoeitic precursor cell-derived mRNAs in plasma exosomes and microvesicles were measured.1

*1
Aoki J et al. Posttransplantation Bone Marrow Assessment by Quantifying Hematopoietic Cell-Derived mRNAs in Plasma Exosomes/Microvesicles. Clinical Chemistry 60:4, 675-682 (2014).

Specifications

System Capacity

  • Up to 96 samples processed per plate

Sample Volume

  • ExoComplete 96-well Plate Kit
    ≤ 400 µL per well

Storage

  • Ambient

Regulatory Approvals

  • For Research Use Only

Disclaimer

The ExoComplete™ 96-Well Plate Kit and ExoComplete™ Tube Kit are Research Use Only Products. Neither of these products are intended for the diagnosis, prevention, or treatment of a disease, nor have they been validated for such use either alone or in combination with other products.