A high-throughput system from extracellular vesicles (EVs) to mRNA capture
Extracellular vesicles (EVs), once thought to be merely waste excreted from cells, are proving to be viable biomarkers and offering new insight into cell-to cell communication in both normal and disease states. EVs are released from cells and found in most human body fluids, including blood, urine, cerebrospinal fluid, and saliva. They encapsulate intracellular proteins, mRNA, and microRNA that can be used to help better understand the functions of cells at the molecular level.
Conventional methods of EV isolation, namely ultracentrifugation, tended to produce clean samples but involved a tedious process not amenable to parallel processing of large sample numbers. Likewise, conventional mRNA purification methods require organic solvent or lengthy washing steps which can introduce error and decrease yield. The ExoComplete™ was designed to overcome these issues. The ExoComplete™ system isolates EVs from biological samples such as plasma in 96-well or 8-well strip filter format and from larger-volume samples such as urine in a novel single collection tube format. A proprietary filter material enables fast filtration of biological samples without clogging and reproducible isolation of EVs without the use of conventional ultracentrifugation After lysing the captured EVs, mRNA can be isolated directly though hybridization with single stranded oligo (dT) immobilized in the wells of mRNA Capture plate or mRNA Capture strip. The advantage of this method over conventional magnetic beads and spin column methods is a seamless process and better consistency between well-to-well and assay-to-assay runs.
EMVs in body fluids are captured either with the Exosome Isolation Plate or the Collection Tube depending upon the sample type. A proprietary, filter material enables fast filtration of biological samples without clogging and reproducible isolation of EMVs without the use of conventional ultracentrifugation.
After lysing the captured EMVs, mRNA can be isolated directly through hybridization with singlestrand oligo (dT) immobilized in the wells of the 96-well mRNA Capture Plate. The advantage of this method over conventional magnet beads and spin column methods is a better consistency between well-to-well and assay-to-assay runs.
The ExoComplete™ series are Research Use Only Products. Neither of these products are intended for the diagnosis, prevention, or treatment of a disease, nor have they been validated for such use either alone or in combination with other products.